Species:

Rat

Alternative Name:

Malate dehydrogenase cytoplasmic,Mdh1,Cytosolic malate dehydrogenase,Mor2

Uniport ID:

O88989

Assay Type:

Sandwich

unit:

pg/mL

Standard:

5000pg/mL

Sensitivity:

46.88 pg/mL

Detection Range:

78.13-5000pg/mL

Assay Time:

3.5H

Sample Type:

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=100μL

Research:

Signal transduction;Cardiovascular biology;Neuro science;Hormone metabolism;

Test Principle:

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MDH1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MDH1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MDH1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MDH1.You can calculate the concentration of Rat MDH1 in the samples by comparing the OD of the samples to the standard curve.